Tuesday, November 26, 2013

Missing In Action

Lately, I’ve been as hard to find as a black panther or wood ape. I apologize for the lack of posts and action on the blog. My teaching and coaching duties are in high gear right now. In addition, my wife is working on her Master’s Degree so I’m having to take on some extra duties around the house in order to help her out.

One exciting thing that has been occupying a lot of my time of late is the impending publication of a children’s book I’ve written. This is my first go around with this sort of thing and, believe me, the writing, editing and publishing process is quite arduous. My hat is off to professional writers and artists. I’ll be giving you some more details on the book as soon as I know exactly when it is going to be available.

I do have some things on the horizon. I hope to get my new camera deployed soon and have some ideas on future posts. It may be after the holidays my schedule eases up enough to get back to full speed, however.

I appreciate all of you who are bearing with me during this, my busiest time of the year.

More soon, I promise.

Monday, November 18, 2013

What Can We Expect To Learn From Wood Ape DNA?

With the cryptozoological community all aflutter over the Bryan Sykes DNA study, I thought now would be a good time to discus what exactly it is we might expect should viable wood ape DNA ever be successfully obtained. I can’t take credit for the question. Fellow NAWAC investigator Ed Harrison posed the question on our online member’s forum. Some of the specific questions raised by Ed were: Will we be able to determine the origins of the wood ape species (geographically)? From which branch of the primate tree did the species spring? How big do they really get? Many other questions were raised and discussed as well but you get the idea.

What we do know is that simply observing/documenting DNA similarities between species says little, or may say very little, about morphological and/or behavioral similarities. A good example of this is the comparison of human and chimp DNA, which shows great overlap.

For example, let’s assume that wood ape DNA is 99% comparable to that of a modern human. That, in and of itself, wouldn't mean they are "human" as we understand the concept. Significant differences could result from factors controlling the expression of genes. The sequence in which genes are activated during development, the duration in which the genes are active, epigenetic factors controlling which genes may be turned off, these factors could produce creatures that are very different from "us" even if the DNA is nearly identical.

Another NAWAC member with great expertise in this area is Brad McAndrews. Brad holds an ABHI certificate from the American Board of Histocompatibility and Immunogenetics and is a Certified Histocompatibility Specialist (also known as a Clinical Histocompatibility Scientist in some states). Brad received his undergraduate degree from the University of Texas at Austin (B.S. in Biology with concentration in Genetics and Biotechnology) and followed that with 5 years of intensive training at a clinical laboratory of the University of Texas Health and Science Center in Houston, TX. All of that to say, Brad knows what he is talking about when it comes to genetics. Following is a summary of Brad’s thoughts to the questions posed above:

"What can we expect to find in the DNA?"

“The study of the wood ape genome will be multifaceted in that several scientists and organizations of varying expertise will study their genes of interest. It will take years to draw lines of correlation between parallel species but science will begin to produce results, the wood ape blueprint, in only days. We will learn about their growth curves, metabolic characteristics, endocrine pathways, and we’ll be able to make discoveries from other genetic linkages already being studied in the higher primates. The endocrine system drives the growth and development cycle from the fetal stage through adolescence (and even adulthood). We'll learn a great deal from studying the endocrine system (which includes hormones, etc) including various physical attributes including relative musculature, to things like emotional states, sleep patterns, and details of the reproductive system. We’ll learn about its dietary capabilities and/or restrictions by studying certain metabolic factors.”

“We will learn details on their ability to learn, hearing, sight, the ability to vocally enunciate (both vowels and consonants) – the FOXP2 gene. We'll learn some interesting things about how they learn, and where their strongest cognitive and/or involuntary cerebral strengths lay. One of the biggest, and most complicated, targets of the wood ape genome will focus on facets of the immune system. Studies of the major histocompatibility complex (MHC) and human leukocyte antigen (HLA) repertoire will teach us a lot about disease susceptibilities and as well as possible new constructs (i.e. mutations) that may help the wood ape fight certain diseases – like Lyme or RMSF. HLA is my particular field of study. No doubt, the discovery of the wood ape will lead to new treatments for mankind. I foresee a day when cells from the wood ape are cloned and distributed (for profit) for study all across the globe. New drugs and therapies will emerge after studying certain aspects of the wood ape immunopathology and it constructs.”

“We’ll also learn many other things that are not specifically related to the wood ape's nuclear or mitochondrial genome. Various microbiotomes will be discovered in the animal's gut flora and new bacterial species/populations may be found on the epidermis (skin) or mouth cavity. We could learn a lot simply by swabbing the cheek of an ape…. We might learn what types of foods they eat even if their entire digestive tract is empty. We can learn about how they metabolize certain foods and compounds.”


“Is the wood ape a descendent of Gigantopithecus blackii? What is the evolutionary lineage?”


“This question will be answered very quickly by sequencing the < 20,000 base pairs of DNA from the mitochondrial genome and comparing that to what data is available for G. blacki. This is easy science. Compare this to all the highly mutagenic interesting stuff found in the more than 3 billion base pairs of the nuclear genome!"


“What can we expect to find within a non-contaminated sample of tissue/blood that has been scientifically proven to be valid?"

“We’ll discover a great many things (as we touched on above). The difficult part of this is that in the scenario where we’ve only a small amount of blood/tissue, proving the existence of this creature as a valid species has limitations. The specimen itself, in this case, is a limiting factor, which could prevent full independent and reproducible study. Basically, science demands that third-party laboratories reproduce the same data set to support the initial find thus creating a “theory.” Ideally, this would include the initial steps of DNA isolation/extraction from the specimen in question. In the case with our Echo samples, we’ve only two very small and degraded residues… That’s huge limitation for us.”

"Will we find the origins of wood apes (geography)?"


“Information on evolutionary history and genetic lineage/origins may be pulled from the mitochondrial genome of a given species. Even without the full mitochondrial genome, much of this information is attainable by analyzing comparative evolution of the mitochondrial cytochrome b gene – a humble 1,200 base pairs of information. Determining geographic origin, however, would require an existing known ancestral “anchor” species in a given geography. When comparisons between two closely related species are being made, this information becomes less reliable because of limitations in sample (that being the gene targeted for sequencing) size. In these cases, additional sequence targets are valuable. Basically, the targeted gene of study may be too highly conserved between the two species. There are only 33 mutations between human and chimp, a mere 2% variation. What might we expect with the wood ape? The comparisons are so alike that it brings into question human contamination… A more likely conclusion to an odd result that “looks human.”

"How far back does the gene pool go (timeline)?"

“A gene pool is actually a collection of all heredities that currently exist in a given and accessible (in regards to breeding) population. That is, how variable (i.e. stable) is the gene pool of the wood ape population? I think what you’re trying to ask is “how far back did this animal branch out from the tree of life?” The answer to this question would be one of the first addressed via the sequencing of the mitochondrial (maternally inherited) genome. Thankfully it would be the easiest of all to answer in terms of testing and it would provide answers to those questions related to its evolution history and origins. This is what I am most curious about personally. I think this is true for many. This is also where Bryan Sykes holds his expertise. A simple science in today’s world.”


"From which branch of the primate tree did the species spring?”


“This will quickly be answered by producing a mitochondrial sequence of the organism. Less than one week of time to produce this data set and a couple-few minutes to run a comparison on GeneBank.”


"How tall [do] these animals really get (physical attributes), etc.?"


"Simply observing/documenting DNA similarities between species says little, or may say very little, about morphological and/or behavioral similarities. A good example of this is the comparison of human and chimp DNA, which shows great overlap."

“While this may certainly be true in a broader context, much of the data derived from genome-wide association studies of similar but different species provide a veritable treasure trove of information that holds value in describing both physical and behavioral phenotypes for a given species. Take a look at the following article, for instance, where it says, “As expected, most of the human genome was closer to the chimp's than to the gorilla's. But in about 15 percent of the genome, human and gorilla resemble each other the most. In another 15 percent, chimp and gorilla DNA are closer to each other than chimp is to human. The analysis also found gene variants in gorillas that are harmless to them but are linked to dementia and heart failure in people. …If we could understand more about why those variants are so harmful in humans but not in gorillas, that would have important medical implications."

"Significant differences could result from factors controlling the expression of genes. The sequence in which genes are activated during development, the duration in which the genes are active, epigenetic factors controlling which genes may be turned off, these factors could produce creatures that are very different from "us" even if the DNA is nearly identical."

“Absolutely spot on here. This is where the revolution in medical genetics (ever heard of this?) will make its biggest strides. Epigenetic gene expressions are influenced by a number of different "micro factors" which may be stimulated by environmental factors and/or stressors. These changes in expression can be either minute or significant. Some changes due to environmental change show themselves phenotypically, while others do not. Many are expressed temporarily, some longer, some for a lifetime, and some are heritable through successive generations (given the continued presence of external pressures). Epigenetic factors, however, are functional in ways that do not cause mutation, or change, to the nucleotide sequence of the organism’s DNA but rather they cause the DNA to be “read” in different ways. This results in coding for different proteins, turning off certain active genes, or by turning on certain dormant ones. Some mechanisms actually modify DNA locally let’s say, for instance, in the epidermis/skin by adding methyl groups to DNA or RNA, or by binding protein to genetic receptors to inactivate a certain from being expressed. This influences the underlying DNA in a way that it is transcribed (or “read”) differently than it was before. You know how some mammals grow thicker coats of fur in the winter and then lose it in the spring? That’s a perfect example of how these factors can influence a change in expression as a product of seasonal changes in temperature (the environmental catalyst). Another fantastic example of epigenetic expression can be seen when a domesticated hog goes feral. Genetic expressions may change very quickly due to environmental stressors, or change. It does not take long before offspring start to resemble feral hogs (the Wild type organism) again with their stunted snouts becoming elongated; hair becoming longer and increasingly coarse, their tusks even grow at faster rates. The domestic pig that gets loose will even begin to show/express “feral” characteristics with longer/thicker hair, increased virility, and quick change in behavior. This is due to something called “phenotypic plasticity,” which is a mechanism that involves a lot of epigenetic factors, caused by environmental alteration or habitat, and which results in changes in genetic expressions – both in phenotype (physical change) and even behavior. The immune system is also greatly influenced by these factors.”

“Anyhow, bottom line is this… just because an organism’s DNA looks similar to another’s from a bird’s-eye view perspective, does not mean that the two organisms are one in the same, or even close to being similar to one another physically, mentally, etc. There is A LOT of stuff going on that goes much deeper than the mere DNA blueprint and we’re just beginning to make sense of it all. We do have a long way to go, sure, but huge strides are being made in the way of knowledge. I can say the following with great confidence, without the detailed genetic comparisons of closely related species, none of these gains in knowledge would be possible.”


Whether the Sykes study yields wood ape DNA remains to be seen. I do think Brad has given us a very good idea of what to expect if and when Sykes, or anyone else, does come up with a viable genetic sample.

One other question sprang to mind when Sykes examined alleged yeti hair and identified it as belonging to a supposedly long extinct species of bear:

"Will mainstream science recognize the existence of a large mammalian species thought to be long extinct based solely on DNA evidence?"

IF the scientific community accepts Sykes’ results and officially recognizes this ancient bear as a living, breathing species that still walks the earth then the “no-kill” crowd may finally have a valid argument as to why a wood ape specimen should not be harvested. If, however, the scientific community refuses to recognize the existence of this ancient bear, a species that they KNOW actually did exist at one point, then there is zero chance that wood ape DNA alone will suffice in documenting the species. If the existence of this ancient bear remains unrecognized after intense DNA study then it should become crystal clear to everyone that the only way to prove the existence of the sasquatch, or wood ape, is by the taking of a holotype. There would simply be no other way.

Contributors: Alton Higgins, Ed Harrison and Brad McAndrews



Sunday, November 17, 2013

Large Black Cat Photographed in Oklahoma?

The photo below of what appears to be a large black cat was sent to me from the guys at Expanded Perspectives (expandedperspectives.com). The only thing they were able to tell me was that the photo came from Oklahoma.


Now, we've been here before so I can't vouch for the authenticity of the photo or the location where it was allegedly captured. I would welcome any additional details if someone out there knows the story behind this photo.

It is intriguing but I have some concerns about the photo. Lots of vertical "seams" that look a bit fuzzy in it. That probably isn't clear but it's the best I can do to explain what I'm seeing. On the other hand, I'm no expert on photo manipulation and it certainly looks like SE Oklahoma so, who knows?

Again, I'd like to hear from anyone that might have the scoop on this one.

Tuesday, November 5, 2013

The Wisdom of Walt Disney

“The difference between winning and losing is most often… not quitting.”

- Walt Disney

Sunday, October 27, 2013

Texas Black Panther Photo Appears to be a Hoax

Well, I can't say I'm surprised...

The photo posted to Facebook last night of a large black cat is real enough but it was not taken anywhere close to Kosse, TX. Hit this link and it will take you to a story on black leopards in Africa which includes this same photo.

Bummer...

Does this dampen my enthusiasm about the possibility of large black North American cats being real? Momentarily maybe but I'll get over it. One hoaxed photo doesn't mean they don't exist. All I have to do is look at the sighting reports that continue to pour in to my site. These folks are seeing something and I just don't believe they are all mistaken or liars.

Onward...

Texas Black Panther Clearly Photographed...Maybe

Below is a photo allegedly taken by a game camera near Kosse, TX.


Unlike many photos that are purported to show black panthers, this one is undeniably a big cat. It is clearly very large and you can see the spots despite the black coat. IF this shot really was captured in Texas then it is a game changer. The photo was brought to my attention by a Facebook follower who came across it on another Facebook page called Big Bucks & Diesel Trucks.

What is interesting about it is how matter-of-factly the photo was received by the site administrator who said, and I’m paraphrasing, “Not a big buck but this would be a hell of a kill.” No amazement that a “black panther” was photographed. No accusations that this could not have possibly been taken in Texas (though at least one commenter did make that statement). It was the type of response that I would expect from a rural Texan who actually lives and spends time in the outdoors.

I left a message on the site asking the person who uploaded the photo to contact me but have not heard from her yet. Keep your fingers crossed that she does and that I can get down there in the near future to investigate.

The photo is clearly genuine. There is no doubt what it shows. What we do NOT know with certainty is where the picture was taken. If there is anyone out there that knows the story on this photo, please contact me at Texascryptidhunter@yahoo.com.

Saturday, October 26, 2013

Black Panther Sighting Distribution Map

Anyone who has read this blog for very long knows of my interest in the cryptid black panthers that many are convinced roam the more remote areas of Texas and the Deep South. I know that there is not supposed to be any such animal. I know that the term black panther is generally considered to be a misnomer and that the only large melanistic cats out there are the leopards of Africa and the jaguars of Central and South America. I know that no melanistic cougars have ever been documented in captivity or the wild. I know all of this.

Yet, the sightings continue to pour in…

I’ve never seen a black panther myself but I know several people who say they have. I would trust these men with my life and believe their stories. I have met and interviewed many others who have had sightings of what can only be described as black panthers. I have no reason to doubt their accounts and detected no signs of deception from them. I’ve also seen a handful of very intriguing photos that, while not conclusive, seem to suggest that a large black cat of some sort just might be out there. I want to prove once and for all that these cats are out there. I want them documented and recognized by science. More than anything, I want to know what they are. New species? North American jaguar? What?

I have been holding onto and publishing the accounts of people who claim to have had encounters with these cryptid cats for several years now. I thought the accounts were fascinating and would make interesting reading for my followers but also hoped that some kind of pattern would become apparent as I studied them. Maybe some common denominator would appear that would help me zero in on where the best place to find one of these animals might be. I wanted to create a distribution map showing the location of each sighting. Only recently did I come to the point where I felt I had enough credible sighting reports to begin the process of creating such a map. I have plotted the location of right at one hundred sightings of large, black, long-tailed cats in the Lone Star State. I’ve included a handful of reports out of Oklahoma that have been sent to me as well. I have received sighting reports from other regions of the country and have published them in various blog posts but they are not included on this particular map. My black panther distribution map is below. The map shows the major rivers of Texas by name. Major cities are denoted by black dots. Sightings of large, black, long-tailed cats are denoted by red dots.


Have any patterns emerged as a result of my effort? Maybe. It appears that there are three definite hot spots for black panther sightings. They are the North Texas area surrounding the D-FW Metroplex, the Texas Hill Country and the Big Thicket/Piney Woods area of Southeast Texas. Central Texas has a high concentration of sightings as well but they are not in quite as tightly packed as the reports coming out of these other three regions. There is also a nice concentration of sightings in Northeast Texas.

Major rivers seem to play an important role in these sighting reports, particularly the areas where the rivers originate. For example, the Hill Country sightings are packed tightly within an area sandwiched between the headwaters of the Nueces, San Antonio, and Guadalupe Rivers. The Trinity River is a major player, too, with sightings concentrated at its beginning point (North Texas) and ending point (Southeast Texas). Secondary hot spots share this characteristic as well. Take the mini-flap of sightings just south of Wichita Falls near the point where the Brazos really begins to take shape and the small concentration of sightings in Northeast Texas near the headwaters of the Sabine and Neches Rivers. One seeming exception to the “headwaters” pattern is in Central Texas. The sightings here are concentrated in the region where the Brazos and Colorado Rivers begin to flow pretty close to one another but are already well established. Smaller rivers like the Leon, Little River, and Lampasas, however, do originate in this area so maybe it isn’t an exception after all. Southeast Texas, though, would definitely be an exception. This is the area where rivers culminate. The Sabine, Neches and Trinity all dump into the Gulf of Mexico near this area. The area does share many similarities with the headwaters areas mentioned above as large numbers of creeks, bayous and marshes cut through the region. These areas are inhospitable to humans but rich in resources for wildlife.

The fact that the sightings are concentrated near and along rivers is certainly no surprise. The waterways are natural corridors that wildlife uses to travel from one area to another. Even rivers that flow through major metropolitan areas, like the Trinity, are often surrounded by greenbelts a mile or more in width. These greenbelts certainly would not sustain a large predator for any real length of time but would provide more than adequate food resources and hiding places for transient cats as they moved through a highly populated area.

What is a bit eye opening to me is how the sightings are bunched up near the headwaters of rivers. I have no real explanation for this. Are these areas somehow richer than other areas along the path of these rivers? Maybe. Certainly the concentration of springs, creeks and small streams in these areas would make for a rich environment. Add the fact that many of the areas where our major rivers originate are still fairly remote and we might be on to something.

Please keep sending in your reports of black panther sightings. Encourage any friends you know that have seen these cats to do the same. The more credible sightings I can plot on the map the better.

Maybe we really can begin to zero in on the mysterious black panthers of Texas.